Search results for "Cerebrospinal Fluid Proteins"

showing 4 items of 4 documents

Analysis of tear proteins by one- and two-dimensional thin-layer iosoelectric focusing, sodium dodecyl sulfate electrophoresis and lectin blotting. D…

1998

· Background: Isoelectric focusing (IEF) of tear proteins has not yet been carried out in a satisfactory way. Two-dimensional (2D) electrophoresis, especially in the combination of IEF with SDS, is able to differentiate between proteins in detail. The purpose of this study was therefore to analyze tear proteins by 1D IEF alone and in combination with a 2D pattern, and by IEF followed by lectin staining. · Methods: Ampholines, covering a broad range from pH 3 to pH 10, were applied. After IEF, semi-dry blotting and incubation with a group II lectin and two group V lectins was performed. · Results: Tear proteins could be separated into 31 single bands. Tear-specific pre-albumin (TSPA), lactof…

AdolescentImmunoblottingCellular and Molecular Neurosciencechemistry.chemical_compoundSurface-Active AgentsLectinsHumansElectrophoresis Gel Two-DimensionalSodium dodecyl sulfateCystatin CEye Proteinschemistry.chemical_classificationbiologyLactoferrinIsoelectric focusingTransferrinLectinSodium Dodecyl SulfateCerebrospinal Fluid ProteinsCystatinsSensory SystemsOphthalmologyLactoferrinIsoelectric pointchemistryBiochemistryTransferrinImmunoglobulin GTearsImmunoglobulin A Secretorybiology.proteinFemaleMuramidaseCystatinLysozymeIsoelectric FocusingGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
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Correlation of the chemiluminescence-activity of peripheral blood monocytes with CSF parameters of inflammation and the clinical course of patients w…

2009

– The chemiluminescence-activity (CL-A) of peripheral blood monocytes (MO) was measured in eight patients with lymphocytic meningitis or meningoencephalitis and compared to CSF parameters and the clinical course. The initial maximum CL-A was around four times above the control and decreased to normal values within approximately 20 days. Poor correlations were found when the CL-A was compared to CSF parameters in the total group of patients. With regard to the CSF parameters in individual patients the CL-A was closely related to the cell count, to a lesser degree to the protein content, but not to the IgG content. Finally, a very good correlation of the CL-A was found with the clinical cours…

AdultMaleAdolescentInflammationMonocyteslaw.inventionProtein contentCorrelationlawMeningoencephalitismedicineHumansMeningitisLymphocytesChildChemiluminescenceCerebrospinal Fluidbusiness.industryClinical courseMeningoencephalitisCerebrospinal Fluid ProteinsGeneral Medicinemedicine.diseasePeripheral bloodLymphocytic meningoencephalitisNeurologyImmunoglobulin GImmunologyLuminescent MeasurementsFemaleNeurology (clinical)medicine.symptombusinessActa neurologica Scandinavica
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Stage-dependent agreement between cerebrospinal fluid proteins and FDG-PET findings in Alzheimer's disease.

2011

Cerebral hypometabolism and abnormal levels of amyloid beta (Aβ), total (t-tau) and phosphorylated tau (ptau) proteins in cerebrospinal fluid (CSF) are established biomarkers of Alzheimer's disease (AD). We examined the agreement between these biomarkers in a single center study of patients with AD of severity extending over a wide range. Forty seven patients (MMSE 21.4 ± 3.6, range 13-28 points) with incipient and probable AD underwent positron emission tomography with [18F]-fluorodeoxyglucose (FDG-PET) and lumbar puncture for CSF assays of Aβ1-42, p-tau181, and t-tau. All findings were classified as either positive or negative for AD. Statistical analyses were performed for the whole samp…

Malemedicine.medical_specialtyPathologyAmyloid betaApolipoprotein E4tau ProteinsNeuropsychological TestsGastroenterologySensitivity and SpecificityCerebrospinal fluidAlzheimer DiseaseFluorodeoxyglucose F18Internal medicinemental disordersmedicineDementiaHumansAgedRetrospective StudiesPsychiatric Status Rating ScalesAmyloid beta-Peptidesmedicine.diagnostic_testbiologyLumbar punctureNeurodegenerationCerebrospinal Fluid ProteinsMiddle Agedmedicine.diseasePeptide FragmentsNeurologyPositron emission tomographyArea Under CurvePositron-Emission Tomographybiology.proteinFemaleNeurology (clinical)Alzheimer's diseasePsychologyCognition DisordersKappaBiomarkersFollow-Up StudiesCurrent Alzheimer research
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Influence of artificial blood contamination of the analysis of cerebrospinal fluid

1981

The influence of artificial blood contamination on the quantitative values obtained in the routine examination of the CSF was investigated. On the basis of dilution series from CSF and blood, the correlation between the number of added erythrocytes and the results of leukocyte counts, protein, glucose and IgG estimation was studied. In addition, the influence of the time between CSF sampling and investigation on erythrocytes and leukocytes, IgG, glucose, pH value and ammonia content in CSF contaminated with blood was investigated. The following data relevant to routine examinations resulted: there are linear correlations between the number of erythrocytes artificially added to the CSF and t…

medicine.medical_specialtyTime FactorsBlood contaminationLeukocyte CountsLeukocyte CountCerebrospinal fluidAmmoniaInternal medicinemedicineHumansCSF albuminCerebrospinal Fluidchemistry.chemical_classificationmedicine.diagnostic_testChemistryLumbar punctureTemperatureCerebrospinal Fluid ProteinsHydrogen-Ion ConcentrationAmino acidDilutionCytolysisBloodGlucoseEndocrinologyNeurologyBlood PreservationImmunoglobulin GImmunologyErythrocyte CountNeurology (clinical)Drug ContaminationJournal of Neurology
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